Astrocyte expression of a dominant-negative interferon-γ receptor
نویسندگان
چکیده
منابع مشابه
Increasing Affinity of Interferon-γ Receptor 1 to Interferon-γ by Computer-Aided Design
We describe a computer-based protocol to design protein mutations increasing binding affinity between ligand and its receptor. The method was applied to mutate interferon-γ receptor 1 (IFN-γ-Rx) to increase its affinity to natural ligand IFN-γ, protein important for innate immunity. We analyzed all four available crystal structures of the IFN-γ-Rx/IFN-γ complex to identify 40 receptor residues ...
متن کاملExpression of a dominant negative mutant of the FGF receptor disrupts mesoderm formation in Xenopus embryos.
Peptide growth factors may play a role in patterning of the early embryo, particularly in the induction of mesoderm. We have explored the role of fibroblast growth factor (FGF) in early Xenopus development by expressing a dominant negative mutant form of the FGF receptor. Using a functional assay in frog oocytes, we found that a truncated form of the receptor effectively abolished wild-type rec...
متن کاملExpression of a dominant-negative mutant human insulin receptor in the muscle of transgenic mice.
To examine the in vivo effects of a kinase-deficient mutant human insulin receptor, we used the muscle creatine kinase promoter to express a putative dominant-negative receptor: Ala1134-->Thr (Moller, D. E., Yokota, A., White, M. F., Pazianos, A. G., and Flier, J. S. (1990) J. Biol. Chem. 265, 14979-14985) in transgenic mice. Two lines were generated, where receptor expression was restricted to...
متن کاملA dominant negative over expression model of mammalian MED12 function
Although schizophrenia has been shown to have a substantial component, there is a dearth of known risk alleles. Furthermore, all of the known risk genes are of small effect sizes. Previously it has been shown that a 12 base pair insertional polymorphism in the in the C-terminal, opposite paired (Opa) domain of the MED12 gene known as MED12 represents a small but significant risk for a positive ...
متن کاملCorrelation between Polymorphism of -56 SNP (T/C) Interferon-γ Receptor 1 Gene and Chronic HBV Infection
Background and Aims: chronic HBV infection is one of the most common viral infections in worldwide which many factors such as genetic factors are involved in pathogenesis of disease. Gamma interferon (IFN-γ) and its receptor (INFGR) play a critical role in the immune response to HBV infection. Single nucleotide polymorphisms (SNPs) are effective on level of gene expression, The aim of thi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Neuroscience Research
سال: 2005
ISSN: 0360-4012,1097-4547
DOI: 10.1002/jnr.20616